Susceptibility of a field-derived, Bacillus thuringiensis-resistant strain of diamondback moth to in vitro-activated Cry1Ac toxin

Article Abstract:

Research has been conducted on the Plutella xylostella, a diamondback moth. The use of the crystalline, solubilized and activated forms of the Bacillus thuringiensis Cry1Ac delta-endotoxin in examining the resistant and susceptible forms of this this moth is described.

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Common, but complex, mode of resistance of Plutella xylostella to Bacillus thuringiensis toxins Cry1Ab and Cry1Ac

Article Abstract:

The genetic and biochemical bases of resistance of Cry1Ab-SEL to Cry1Ab are studied and these are compared to the nature of Cry1Ac resistance in CryAc-SEL. Results reveal that resistance to Cry1Ab in Cry1Ab-SEL is similar to Cry1Ac in Cry1Ac-SEL.

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A change in a single midgut receptor in the diamondback moth (Plutella xylostella) is only in part responsible for field resistance to Bacillus thuringiensis subsp. kurstaki and B. thuringiensis subsp. aizawai

Article Abstract:

Two subpopulations of diamondback moths (SERD3) exhibiting resistance to Bacillus (B.) thuringiensis subsp. aizawai and B. thuringiensis subsp. kurstaki were analyzed to determine the mechanisms of microbial resistance. Analysis of the Plutella xylostela SERD3 subpopulations indicated the presence of receptor-independent, unstable resistance mechanisms and the absence of cross-resistance to B. thuringiensis insecticidal crystal proteins. Furthermore, selection experiments confirmed the presence of genetic variation that mediates resistance to B. thuringiensis subspecies.

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