Article Abstract:
Primers based on the Hip1 sequence have been used as a typing technique for cyanobacteria. PCR-based methods for typing have been developed over the years. An octameric palindromic sequence Hip1 is present in the chromosomal DNA of many cyanobacteria. PCR primers were used to extend the Hip1 sequence and five produced different products when used to prime PCR from cyanobacterial genomic DNA. RFLP and Hip1 typing produced similar results and each technique could distinguish different strains.
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Article Abstract:
Thermomonospora species were grown on a mixture of cellulose and starch in mineral salts minimal medium to understand thermophilic actinomycetes' inducer control over biosynthesis of unusual alpha-amylase. Results show that cellulose or cellobiose blocks induction of alpha-amylase via inducer exclusion and initiates inactivation of the amylase via proteolysis. It was also found that the rapid decrease of alpha amylase activity is not influenced by thermal instability of the enzyme.
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Article Abstract:
Research was conducted to examine the 10 Staphylococcus caprae strains isolated from four patients that were suffering from joint and bone infections following the implantation of orthopaedic material. The objective was to characterize the strains as well as four unrelated S. caprae strains isolated from the milk of mastitis-free goats. Results reveal atlE and ica genes in goat and human strains of S. caprae.
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