A PCR detection method for rapid identification of Paenibacillus larvae

Article Abstract:

A polymerase chain reaction (PCR) detection method which can be used on colonies that grow on this semiselective medium is described. The PCR method allows the rapid confirmation of the existence of paenibacillus larvae. Rapid conformation of the presence of Paenibacillus larvae is prevented by false-positive colonies growing on the bacterium. PCR primers are designed based on the 16S rRNA gene of Paenibacillus larvae and selectively amplify a 973-bp amplicon.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Characterization of bacterial and fungal soil communities by automated ribosomal intergenic spacer analysis fingerprints: biological and methodological variability

Article Abstract:

Automated rRNA intergenic spacer analysis (ARISA) can be used to analyze microbial communities. Researchers used ARISA to analyze the 16S-23S intergenic spacer region from the bacterial rRNA operon and the two internal transcribed spacers and the 5.8S rRNA gene from the fungal rRNA operon.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Rapid approach to determine rrn arrangement in Salmonella serovars

Article Abstract:

Researchers have designed PCR primers for the regions surrounding the seven rrn operons of Salmonella enterica serovars. The rrn operons are the source of chromosomal rearrangements in bacteria.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code: