Article Abstract:
A 13 kb resolution cosmid map is presented for the 14 Mb fission yeast genome. This technique is advantageous over others since its utilization of nonrandom sequence-tagged site (STS) probes allows it to function not only as templates for DNA sequencing but also as a public data bank for accessing DNA at any region for gene cloning and mapping procedures. Other advantages are present. The technique is also an optimized version of random STS content mapping since it minimizes redundant work at every stage. However, various issues are discussed regarding the map's potential for mammalian genomes.
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Article Abstract:
The genes that mediated sister chromatid sepparation in centromeres were analyzed during mitosis in temperature-sensitive yeast mutants that carry a minichromosome. Mutants that lose the minichromosome at the permissive or semipermissive temperatures exhibited reduced viability due to alterations in the expression of mis6+. Disruptions in the chromosomal mis6+ gene also resulted in the unequal segregation of the chromosomes in yeast cells.
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Article Abstract:
A mutation of the fission yeast protein, Mis4, may form a link between chromatids in S phase that splits rather than being removed in anaphase. Research with Mis4 shows it is a different chromatid cohesion molecule, independent of cohesin, and can pass from yeast to human. Mis4 is neither essential for condensation and does not degrade in phase G(sub)1.
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