4-methylphthalate catabolism in Burkholderia (Pseudomonas) cepacia Pc701: a gene encoding a phthalate-specific permease forms part of a novel gene cluster

Article Abstract:

The nucleotide sequence of a 4.4 kbp fragment of pMOP, a plasmid which is essential in 4-methylphthalate metabolism in Burkholderia cepacia Pc701, was identified. Two complete ORFs were determined and called mopA and mopB. mopB encodes a 4-methylphthalate permease which belongs to a superfamily of symport proteins found in both prokaryotes and eukaryotes. The predicted protein MopA indicate substantial homology to reductase proteins involved in aromatic and aliphatic metabolism, and had motifs recognized as binding the ADP and the flavin moieties of FAD/NAD.

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The use of two-dimensional gradient plates to investigate the range of conditions under which conjugal plasmid transfer occurs

Article Abstract:

Gel-stabilized two-dimensional gradient plate system is very effective in studying the influence of pH, temperature and salt concentration on conjugal plasmid, RP4, transfer among strains of Escherichia coli and Pseudomonas putida. The time, temperature, the donor and recipient bacteria and the combination of donor and recipient bacteria affect the size and shape of the conjugation domain. At low salt concentration the pH range of conjugation is wide and with rises in concentration the range decreases.

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Comamonas acidovorans UCC61 catabolizes o-phthalate via a 4,5-oxygenation pathway that encoded on a 70kbp section of plasmid pOPH1 bounded by directly repeated sequences

Article Abstract:

A Comamonas acidovorens strain bacteria, UCC61, isolated from sewage sludge catabolizes o-phthalate to protocatechuate by 4,5-oxygenation, dehydrogenation and decarboxylation indicating that the 270 kbp plasmid pOPH1 region of the contains the phthalate-degrading genes. Elimination of Pht element results in complete loss of Pht+ phenotype.

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