3-methylindole (skatole) and indole production by mixed productions of pig fecal bacteria

Article Abstract:

L-tryptophan conversion to indole or 3-methylindole (skatole) by the bacterial population in the large intestines of pigs was examined. In vitro study of pig fecal slurries revealed an indole-3-acetate intermediate in the production of 3-methylindole and a highest rate of production at pH 6.5. At pH 5.0, 3-methylindole was the product of 80% of tryptophan conversion. At pH 8.0, indole constituted 85% of the product. The two pathways had similar maximum rates and tryptophan Km values.

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Cloning of the trp gene cluster from a tryptophan-hyperproducng strain of Corynebacterium glutamicum: identification of a mutation in the trp leader squence

Article Abstract:

Tryptophan biosynthesis gene clusters of the Corynebacterium glutamicum strain were cloned and characterized from Escherichia coli. Resistance of E. coli strains to tryptophan logs was demonstrated. A comparison is made between the amino acid sequence deduced by trpe gene with those encoded from other bacteria. Results indicate the occcurrence of a nonsense mutation which causes constitutive antitermination response.

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Acid tolerance of biofilm cells of Streptococcus mutans

Article Abstract:

The acid tolerance response (ATR) and ATR-inducing ability of the biofilm cells of Streptococcus mutans are explained. The results show that different strains of Streptococcus mutans possess different degrees of acid tolerance and ability to induce ATR.

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