3-hydroxypropionaldehyde, an inhibitory metabolite of glycerol fermentation to 1,3-propanediol by enterobacterial species

Article Abstract:

The fermentation of glycerol to 1,3-propanediol by Enterobacter agglomerans produces 3-hydroxypropionaldehyde as an intermediate product that inhibits the formation of 1,3-propanediol. The synthesis of 1,3-propanediol stops after 430 mM of glycerol have been utilized by the enterobacterial species regardless of the initial glycerol concentration. 3-hydroxypropionaldehyde is also formed during the fermentation of glycerol by other enterobacterial species such as Klebsiella pneumoniae and Citrobacter freundii.

author: Soucaille, Philippe, Barbirato, Fabien, Grivet, Jean Philippe, Bories, Andre
Microbial metabolism, Enterobacteriaceae, Fermentation

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Modulation of carbon and electron flow in Clostridium acetobutylicum by iron limitation and methyl viologen addition

Article Abstract:

Examination of the metabolism of Clostridium acetobutylicum as a function of methyl viologen addition and iron limitation shows that both factors exhibit ability to induce metabolic shifts toward butanol formation. Additive physiological effects of these factors reveal different and complementary sites of action for these factors. Addition of methyl viologen increases the activity of C. acetobutylicum and generates an artifical electron transport chain.

author: Soucaille, Philippe, Peguin, Sophie
Clostridium, Biological transport, Iron, Iron (Metal)

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Microbial conversion of glycerol to 1, 3-propanediol: Physiology comparison of a natural producer, Clostridium butyricum VPI 3266, and an engineered strain, Clostridium acetobutylicum DGI (pSPD5)

Article Abstract:

A study is conducted on the comparative physiological behavior of the recombinant Clostridium bacetobutylicum DG1(pSPD5) strain with that of the natural 1,3-propanediol producer Clostridium butyricum VPI 3266, by growing both the strains in chemostat cultures with glycerol as the sole carbon source. It is observed that both the strains showed the same 'global behavior'.

author: Soucaille, Philippe, Meynial-Salles, Isabelle, Gonzalez-Pajuelo, Maria, Mendes, Filipa, Vasconcelos, Isabel
Science & research, All Other Basic Organic Chemical Manufacturing, CHEMICALS AND ALLIED PRODUCTS, Glycerine, Microbiological synthesis, Clostridium botulinum, Nucleic acids, Glycerol, Chemical properties, Glycerin, Nucleic acid isolation

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subjects list: Research, Analysis, Physiological aspects
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