Article Abstract:
A polymerase chain reaction-based method for the rapid detection of Listeria monocytogenes was developed. The procedure involved the use of primers based on a unique region in the 16S rRNA sequence of L. monocytogenes. The procedure resulted in shorter denaturing and annealing times, as well as more rapid transitions, to detect as few as 2 to 20 colony forming units of L. monocytogenes in inoculated food samples. Further analysis showed that the procedure is highly specific for L. monocytogenes, although soft cheese produced interference.
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Article Abstract:
Two pairs of primers were designed for use in a ligase chain reaction (LCR) assay to discriminate Listeria monocytogenes from other Listeria species. The primers were based on the 16s rRNA gene containing a single base-pair difference in theV9 region. Prior to LCR, the probes were amplified using the polymerase chain reaction. Tests on 19 different Listeria species showed that the procedure could effectively discriminate between species, and can therefore be a useful diagnostic tool for detecting Listeria monocytogenes.
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Article Abstract:
A PCR procedure based on 16S rRNA gene sequencing was used to detect 12 anaerobic bacteria in human and animal feces. The technique, which is simple and quick, is specific for these anaerobic bacteria that occur in human intestinal tracts. The results of the procedure compare well with those obtained from the usual more tedious procedures for the detection of the bacteria.
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